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| BIO 532 | Research Techniques in Biochemistry - II | 3+0+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of MEDICAL BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | -- | | Co-Lecturer | Assoc. Prof. Dr. Ahmet ALVER | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The course aims to provide the students with a general knowledge on the theory of techniques used in biochemistry |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | know biochemical methods | 1,2,3,4 | 1,3 | | PO - 2 : | learn biochemical laboratory methods in human health | 1,2,3,4 | 1,3 | | PO - 3 : | apply biochemical methods | 1,2,3,4 | 1,3 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Principles of Chromatography Elution Methods TLC/Paper Chromatography Ion-Exchange Chromatography Gel Filtration Affinity Chromatography Hidrofobic Interaction Chromatography Principles of Electrophoresis Support Media Polyacrilamide gel (SDS-PAGE) Blotting Protein Electrophoresis in Clinical Diagnosis |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Definitions/Concepts of Proteomics | | | Week 2 | Elution Methods | | | Week 3 | TLC/Paper chromatography/HPLC | | | Week 4 | Ion-Exchange Chromatography | | | Week 5 | Gel filtration | | | Week 6 | Affinity Chromatography | | | Week 7 | Hidrofobic Interaction Chromatography | | | Week 8 | Mid-term exam | | | Week 9 | Principles of Electrophoresis | | | Week 10 | Support media | | | Week 11 | Polyacrilamide gel (SDS-PAGE) | | | Week 12 | Continuous/Discontinuous Systems /Homework | | | Week 13 | Blotting | | | Week 14 | Protein Electrophoresis in Clinical Diagnosis | | | Week 16 | End-of-term exam | | | |
| 1 | Robyt,JF., White BJ.1987;Biochemical Techniques,Waveland Press, Illinois | | | 2 | Boyer, RF.1993;Modern Experimental Biochemistry, The Benjamin /Cummings Publ.,California | | | 3 | Barker,K.1998;At The Bench, Cold Spring harbor Lab Press, New York | | | |
| 1 | Pingoud,A.,Urbanke,C,Hoggett,J.Jeltsch,A.2002; Biochemical Methods, Wiley-VCH,Weinheim | | | 2 | Metzler, D. 2001; Biochemistry, Vol.1, Harcourt-Academic Press,San Diego | | | 3 | Metzler, D. 2003; Biochemistry, Vol.2, Harcourt-Academic Press,San Diego | | | 4 | Barker,K.1998;At The Bench, Cold Spring harbor Lab Press, New York | | | 5 | Roskams,J., Rodgers,L.2002;Lab Ref,Cold Spring harbor Lab Press, New York | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | | 2 | 30 | | Homework/Assignment/Term-paper | 12 | | 4 | 20 | | End-of-term exam | 17 | | 3 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 6 | 14 | 84 | | Laboratuar çalışması | 0 | 0 | 0 | | Arasınav için hazırlık | 3 | 14 | 42 | | Arasınav | 2 | 1 | 2 | | Uygulama | 0 | 0 | 0 | | Klinik Uygulama | 0 | 0 | 0 | | Ödev | 6 | 2 | 12 | | Proje | 0 | 0 | 0 | | Kısa sınav | 0 | 0 | 0 | | Dönem sonu sınavı için hazırlık | 2 | 14 | 28 | | Dönem sonu sınavı | 3 | 1 | 3 | | Diğer 1 | 0 | 0 | 0 | | Diğer 2 | 0 | 0 | 0 | | Total work load | | | 213 |
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