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| TBI5064 | Protein Methods | 2+0+2 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of MEDICAL BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Group study, Lab work , Practical | | Contact Hours | 14 weeks - 2 hours of lectures and 2 hours of laboratory per week | | Lecturer | Doç. Dr. Tuba DİNÇER | | Co-Lecturer | Gökhan Yıldız, Ersan Kalay | | Language of instruction | | | Professional practise ( internship ) | None | | | | The aim of the course: | | Aim of the course is to provide students with understanding and applications of general methods to analyse functions of protein. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Identification of protein 3-D structure, conserved motifs and domains by using protein based computer programmes | | 5 | | PO - 2 : | Expression of protein of interest in bacteria, yeast and mammalian cells | | 4 | | PO - 3 : | Purification of protein of interest | | 1,3,4 | | PO - 4 : | Analysis of protein of interest | | 1,3,4,5 | | PO - 5 : | Analysis of interaction of protein of interest with other proteins. | | 1,2,4 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| With this course identification of the function of protein will be provided with topics such as; computational analysis of protein using online sources, protein production , purification, visualization and identification of protein of interest interacting proteins. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | General information about protein basics such as structure and function | | | Week 2 | Functional Domains of Proteins | | | Week 3 | Identification of clues about protein function by computer based analysis of protein sequence | | | Week 4 | Expression of protein of interest in bacteria, yeast and mammalian cells. | | | Week 5 | Techniques used for purification of proteins | | | Week 6 | Analysis of purified protein on protein gel with immunoblot technique | | | Week 7 | Analysis of protein cellular location by flourescence microscope based methods | | | Week 8 | Midterm | | | Week 9 | Analysis of post-translational modifications of proteins | | | Week 10 | Identification of protein interacting proteins | | | Week 11 | Methods to Verify Protein Interaction | | | Week 12 | DNA-Protein interaction | | | Week 13 | Assays for definition of protein function, student seminers | | | Week 14 | Introduction to Proteomics, student seminers | | | Week 15 | student seminers | | | Week 16 | Final sınavı | | | |
| 1 | Gwen Taylor, Current Protocols in Protein science, John Wiley and sons, Inc, 2003 | | | |
| 1 | Gregory A petsko and Dogma Ringe, Protein Structure and Function, New Science,2004 | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | | 2 | 30 | | Quiz | 4
10 | | 1 | 10 | | Presentation | 13
14
15 | | 3 | 10 | | Homework/Assignment/Term-paper | 14 | | 5 | 10 | | End-of-term exam | 16 | | 2 | 40 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 14 | 28 | | Sınıf dışı çalışma | 2 | 14 | 28 | | Laboratuar çalışması | 2 | 14 | 28 | | Arasınav için hazırlık | 2 | 7 | 14 | | Arasınav | 2 | 1 | 2 | | Ödev | 2 | 3 | 6 | | Kısa sınav | 1 | 2 | 2 | | Dönem sonu sınavı için hazırlık | 2 | 7 | 14 | | Dönem sonu sınavı | 2 | 1 | 2 | | Total work load | | | 124 |
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