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| TBI5058 | Methods in Molecular Genetics | 2+0+2 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of MEDICAL BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Lab work , Practical | | Contact Hours | 14 weeks - 2 hours of lectures and 2 hours of laboratory per week | | Lecturer | Dr. Öğr. Üyesi Bayram TORAMAN | | Co-Lecturer | ASSIST. PROF. DR. TUBA DİNÇER, | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The aim of this cours is to provide information to students about molecular genetic methods and basic mechanism of this methods. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | use laborotory and laborotory equepments in a molecular genetic laborotory. | 16 | 1,3 | | PO - 2 : | carry out basic molecular genetic methods | 14 | 1,3,4 | | PO - 3 : | explain the mechanism of molecular genetic methods. | 14 | 1,3 | | PO - 4 : | sellect correct molecular genetic methods in a Project | 14,19 | 1,3 | | PO - 5 : | do cell Culture | 15 | 1,4 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| 1. Laboratory using and sterilization
2. DNA and RNA isolation
3. Agarose and pPolyacrylamide gel electrophoresis
4. Polymerase chain reaction (PCR)
5. RT-PCR
6. Mutation screening methods; RFLP, ARMS, SSCP, DGGE, Heterodupleks analysis
7. DNA sequencing
8. Southern Blot
9. Cloning
10. Cell culture
11. Yeast two Hybrid (Y2H)
12. EBV (Ebstein-Bar Virus) transformation
13. GST Pull down |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Laboratory use and sterilisation | | | Week 2 | DNA and RNA isolation | | | Week 3 | Agarose and polyacrylamide gel electrophoresis
| | | Week 4 | Polymerase chain reaction (PCR) | | | Week 5 | RT-PCR | | | Week 6 | Mutation screening methods; RFLP, ARMS, SSCP, DGGE, Heterodupleks analysis | | | Week 7 | DNA sequencing | | | Week 8 | Mid-term exam | | | Week 9 | Southern blot | | | Week 10 | Cloning | | | Week 11 | Cell culture | | | Week 12 | Yeast two Hybrid (Y2H) | | | Week 13 | EBV (Ebstein-Bar Virus) transformation | | | Week 14 | GST Pull down | | | Week 16 | End-of-term exam | | | |
| 1 | Sambrook J, Russell D. 2001; Molecular Cloning: A Laboratory Manual (Third Edition, Cold Spring Harbor Laboratory Pres, Cold Spring Harbor, New York | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | | 2 | 30 | | Homework/Assignment/Term-paper | 12 | | 4 | 20 | | End-of-term exam | 16 | | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 14 | 28 | | Sınıf dışı çalışma | 0 | 0 | 0 | | Laboratuar çalışması | 2 | 14 | 28 | | Arasınav için hazırlık | 7 | 7 | 49 | | Arasınav | 3 | 1 | 3 | | Uygulama | 0 | 0 | 0 | | Klinik Uygulama | 0 | 0 | 0 | | Ödev | 11 | 5 | 55 | | Proje | 0 | 0 | 0 | | Kısa sınav | 0 | 0 | 0 | | Dönem sonu sınavı için hazırlık | 7 | 7 | 49 | | Dönem sonu sınavı | 3 | 1 | 3 | | Diğer 1 | 0 | 0 | 0 | | Diğer 2 | 0 | 0 | 0 | | Total work load | | | 215 |
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