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| TBI5053 | Molecular Genetic Methods | 2+2+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Compulsory | | Department | DEPARTMENT of MEDICAL BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Lab work | | Contact Hours | 14 weeks - 2 hours of lectures and 2 hours of practicals per week | | Lecturer | Dr. Öğr. Üyesi Gökhan YILDIZ | | Co-Lecturer | None | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Basic methods used in molecular genetics |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | learn laboratory equipments | 16 | 1 | | PO - 2 : | Cell seperation and cell culture | 15 | 1,4 | | PO - 3 : | identify DNA and RNA isolatio | 1 | 1,4 | | PO - 4 : | use molecular genetic methods | 14 | 1,3,6 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| DNA and RNA isolationPolymerase chain reactionGel electrophoresisRestriction enzyme analysisDNA sequencingCloningCell culture (Eukaryotic and Prokaryotic) EBV (Ebstein-Bar Virus) transformation) Yeast two HybridGST Pull down |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Design of molecular genetic laboratories | | | Week 2 | Cell separation | | | Week 3 | Cell and tissue culuture methods
| | | Week 4 | DNA and RNA isolation | | | Week 5 | Gel electrophoresis | | | Week 6 | DNA sequencing | | | Week 7 | Southern blotting | | | Week 8 | Mid-term exam | | | Week 9 | Western Blotting | | | Week 10 | Genomics and Proteomics | | | Week 11 | Flow cytometry | | | Week 12 | Real-Time PCR | | | Week 13 | Molecular genetic tests in hemato-oncology | | | Week 14 | Tissue Typing tests | | | Week 16 | End-of-term exam | | | |
| 1 | Rapley, R. Walker, JM. 1998; Molecular Biomethods Handbook, Humana Press, Totowa New Jersey | | | |
| 1 | Rapley, R. 2000; The Nucleic Acid Protocols Handbook, Human Press, Totowa New Jersey | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 1 | 8 | 1,5 | 30 | | Homework/Assignment/Term-paper | 1 | 14 | 0,5 | 20 | | End-of-term exam | 1 | 16 | 1,5 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 12 | 24 | | Sınıf dışı çalışma | 0 | 0 | 0 | | Laboratuar çalışması | 2 | 12 | 24 | | Arasınav için hazırlık | 4 | 6 | 24 | | Arasınav | 3 | 1 | 3 | | Uygulama | 5 | 12 | 60 | | Klinik Uygulama | 0 | 0 | 0 | | Ödev | 3 | 5 | 15 | | Proje | 5 | 7 | 35 | | Kısa sınav | 0 | 0 | 0 | | Dönem sonu sınavı için hazırlık | 6 | 6 | 36 | | Dönem sonu sınavı | 3 | 1 | 3 | | Diğer 1 | 0 | 0 | 0 | | Diğer 2 | 0 | 0 | 0 | | Total work load | | | 224 |
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