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| KIMI7317 | Combined Chromatographic Bioactivity Methods | 3+0+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Third Cycle | | Status | Elective | | Department | DEPARTMENT of CHEMISTRY | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Prof. Dr. Murat KÜÇÜK | | Co-Lecturer | None | | Language of instruction | | | Professional practise ( internship ) | None | | | | The aim of the course: | | Learning combined chromatography ? bioactivity methods that can be used in various research fields, evaluation of their applicability in the local laboratories, gaining practice with current applications. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | learn the basics and terminology of chromatography. | | | | PO - 2 : | learn the basics and terminology of bioactivity. | | | | PO - 3 : | learn advantages and disadvantages of combined chromatography-bioactivity methods. | | | | PO - 4 : | learn the applicability of combined chromatography-bioactivity methods in different fields. | | | | PO - 5 : | learn how to develop a new derivatization method. | | | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Principles and types of chromatography, the material and instruments used, analyses of primary and secondary metabolites and other biomolecules with chromatographic methods, types of bioactivities investigated, current classical bioactivity tests, adaptation of bioactivity methods to chromatographic working systems, current studies of bioactivity measurements based on chromatographic analyses, advantages and disadvantages of the combined applications of bioactivity and chromatography, on-line bioactivity ? HPLC applications, bioactivity ? TLC applications, examples from current literature. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Principles of chromatography | | | Week 2 | Types of chromatography | | | Week 3 | Materials and instruments used in chromatography | | | Week 4 | Analyses of primary and secondary metabolites and other biomolecules with chromatographic methods | | | Week 5 | The bioactivities investigated | | | Week 6 | Current classical bioactivity investigations | | | Week 7 | Adaptations of bioactivity methods into chromatography systems | | | Week 8 | Current bioactivity measurements based on chromatographic analysis | | | Week 9 | Mid-term exam | | | Week 10 | Advantages and disadvantages of combined chromatography ? bioactivity applications | | | Week 11 | On-line biyoaktivite ? HPLC uygulamaları | | | Week 12 | On-line biyoaktivite ? HPLC uygulamaları | | | Week 13 | Bioactivity ? TLC applications | | | Week 14 | Timely examples about literature on combined chromatography-bioactivity methods | | | Week 15 | Recent examples about literature on combined chromatography-bioactivity methods | | | Week 16 | End-of-term exam | | | |
| 1 | Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch, 1997; Practical HPLC Method Development, 2nd Edition, Wiley-Interscience, A.B.D.. | | | |
| 1 | Yuri V. Kazakevich, Rosario LoBrutto, 2007; HPLC for Pharmaceutical Scientists, Wiley-Interscience, A.B.D.. | | | 2 | I. N., Ph.D. Todorov G. E. Zaikov I. A. Degterev, 1992; Bioactive Compounds: Biotransformation and Biological Action, Nova Science Pub Inc. | | | 3 | Jeffrey B. Harborne (Editor), 1998; Phytochemical Dictionary: A Handbook of Bioactive Compounds from Plants, Taylor and Francis, Inc. | | | 4 | Atta-Ur-Rahman (Editor), 2003; Bioactive Natural Products, Vol. 29, Elsevier Science and Technology Books. | | | 5 | D.S. Bhakuni D.S. Rawat, 2005; Bioactive Marine Natural Products, Springer. | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 09/04/2020 | 2 | 30 | | Project | 13 | 07/05/2020 | 2 | 10 | | Presentation | 14 | 14/05/2020 | 2 | 10 | | End-of-term exam | 16 | 09/06/2020 | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 7 | 14 | 98 | | Arasınav için hazırlık | 10 | 1 | 10 | | Arasınav | 2 | 1 | 2 | | Ödev | 10 | 3 | 30 | | Proje | 2 | 2 | 4 | | Dönem sonu sınavı için hazırlık | 12 | 1 | 12 | | Dönem sonu sınavı | 2 | 1 | 2 | | Total work load | | | 200 |
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