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| KIML7780 | Thin Layer and Column Chromatography | 3+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Third Cycle | | Status | Elective | | Department | DEPARTMENT of CHEMISTRY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Practical | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | -- | | Co-Lecturer | Assoc. Prof. Hacer BAYRAK | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Thin layer and column chromatography description, theoretical knowledge, in which mixtures can be applied for, how to apply, techniques, which equipment should be used (chemical or consumption), column fillers (including the preparation of TLC plates), solvent selection and the importance of the solvent selection, Rf definition, and purpose of the column chromatography and aims to gain the practical skills to perform the TLC reviewed. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Reaksiyon süreleri, karışım varlığı ve ayırma gibi yöntemleri öğrenerek daha ileri yapı aydınlatma çalışmalarında saf maddenin önemini kavrayarak güvenilir sonuçlar elde edecek. | | | | PO - 2 : | Students will learn the application and purification techniques of column chromatography. | | | | PO - 3 : | Students will learn and understand the importance of the choice of reaction solvent or purification on Column Chromatography and TLC to obtain accurate results. | | | | PO - 4 : | Student will learn the method of separation mixtures by column chromatography and determine the reaction mixtures etc. by thin layer chromatography and will be aware of pure substance to obtain reliable results for the elucidation further studies. | | | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Thin layer and column chromatography description, theoretical knowledge, in which mixtures can be applied for, how to apply, techniques, which equipment should be used (chemical or consumption), column fillers (including the preparation of TLC plates), solvent selection and the importance of the solvent selection, Rf definition, and purpose of the column chromatography and aims to gain the practical skills to perform the TLC reviewed. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Introduction of Thin Layer Chromatography (TLC), definition and types, use and purpose, advantages and disadvantages. | | | Week 2 | The importance of selection solvent or solvent mixtures using in TLC plates. | | | Week 3 | The interpretation and evaluation of TLC according to the intended use and purpose. | | | Week 4 | Preparation TLC plates in the laboratory (on thin and thick glass plates). | | | Week 5 | The study on the prepared samples on thin and thick glass TLC plates. | | | Week 6 | The definition, types and purpose of Column Chromatography. | | | Week 7 | Column Chromatography fillers and their properties, preparation techniques and tricks. | | | Week 8 | Procedure for preparing the substance mixture to the column. | | | Week 9 | Mid-term exam | | | Week 10 | The importance of solvent or solvent mixture selection in column chromatography. | | | Week 11 | The importance of solvent or solvent mixture selection in column chromatography. | | | Week 12 | Risk factors and safety measures during column chromatography application. | | | Week 13 | Column preparation and purification of substance mixtures (Application in laboratory). | | | Week 14 | Column preparation and purification of substance mixtures (Application in laboratory). | | | Week 15 | Column preparation and purification of substance mixtures (Application in laboratory). | | | Week 16 | End-of-term exam | | | |
| 1 | Dayton, C., 2015, Column Chromatography. | | | 2 | Fried, B; Sherma, J., 2003, Handbook of Thin-Layer Chromatography, CRC Press. | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | | | 50 | | End-of-term exam | 16 | | | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | | | | |
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