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| BIY5270 | Virological Diagnosis | 3+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Prof. Dr. İsmail DEMİR | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The main objectives of this course are to provide the students basic informations about cell culture and virological methods |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | have knowledge about virological diagnosis in detail | 1,3 | 1,2 | | PO - 2 : | do detail literature research on a specific virological methods | 1,3 | 3 | | PO - 3 : | have information about applications of specific and current techniques in virological diagnosis | 1,3 | 4 | | PO - 4 : | use current techniques in virology | 1,3 | 2,4 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Importance of virological methods, Virus isolation, microscopy, complement fixation, haemagglutination, enzyme linked immunoassay, immunofluorescense, radioimmunoassay system, neutralization, assays of viral nucleic asids. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | History, development and importance of tissue culture. | | | Week 2 | Biology of cultured cells and the culture environment. | | | Week 3 | Laboratory equipment and materials and cell culture techniques. | | | Week 4 | Disaggregation of the tissue and characterization of cell lines. | | | Week 5 | Cloning and selection of the specific cell types. | | | Week 6 | Study of viruses in cell culture. | | | Week 7 | Virus replication and stock preparation.Virus production methods | | | Week 8 | Techniques in diagnostic virology. | | | Week 9 | Mid-term exam | | | Week 10 | Use of radioactive isotopes in cell culture. | | | Week 11 | Production of monoclonal antibodies. | | | Week 12 | Presentation | | | Week 13 | Presentation | | | Week 14 | Presentation | | | Week 15 | Current techniques in cell culture | | | Week 16 | End-of-term exam | | | |
| 1 | Freshney, R.I. 2005; Culture of Animal Cells: A Manual of Basic Technique, Wiley-Liss, USA. | | | |
| 1 | Mani, S., Singh, M., Kumar, A., 2023; Animal Cell Culture: Principles and Practice, Springer, USA. | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 21/04/2024 | 1 | 30 | | Presentation | 11
12
13 | 04/05/2024
11/05/2024
18/05/2024 | 0,3 | 20 | | End-of-term exam | 16 | 16/06/2024 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 3 | 14 | 42 | | Laboratuar çalışması | 0 | 0 | 0 | | Arasınav için hazırlık | 2 | 8 | 16 | | Arasınav | 1 | 1 | 1 | | Uygulama | 0 | 0 | 0 | | Klinik Uygulama | 0 | 0 | 0 | | Ödev | 2 | 9 | 18 | | Proje | 1 | 10 | 10 | | Kısa sınav | 0 | 0 | 0 | | Dönem sonu sınavı için hazırlık | 5 | 15 | 75 | | Dönem sonu sınavı | 1 | 1 | 1 | | Diğer 1 | 2 | 10 | 20 | | Diğer 2 | 0 | 0 | 0 | | Total work load | | | 225 |
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