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| MBGL5042 | The Biology of Plasmids and Vectors | 3+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Doç. Dr. Halil İbrahim GÜLER | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The aim of this course is to provide biology and molecular biology students, as well as other life sciences and bioengineering students with no biological origin, to have knowledge about plasmid structure and vector construction, information about the basic principles of choosing a cloning or expression vector, classification of plasmids, replication types, basic functions of plasmids and vectors, and how students can start a new expression study are the objectives of the courses. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Basic functions of plasmids and vectors | 2,5 | 1, | | PO - 2 : | Plasmid replication and control | 2,5 | 1, | | PO - 3 : | Classification of plasmids and vectors | 2,5 | 1, | | PO - 4 : | Expression systems and pET vectors | 2,5 | 1, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Content of the course, classification of plasmids, plasmid replication and control, replication origin types, organization and structure of plasmids, and important plasmid functions. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Anatomy of bacterial plasmids | | | Week 2 | Classification of plasmids | | | Week 3 | Organization and structure of plasmids | | | Week 4 | Important plasmid functions | | | Week 5 | cryptic plasmids | | | Week 6 | Plasmid replication and control | | | Week 7 | Replication origin types | | | Week 8 | Mid-term exam | | | Week 9 | Replication control strategies | | | Week 10 | incompatiblity | | | Week 11 | first vectors | | | Week 12 | E.coli vectors | | | Week 13 | Choosing a cloning vector | | | Week 14 | pET series vectors | | | Week 15 | Popular expression vectors | | | Week 16 | Final exam | | | |
| 1 | Plasmids: A Practical Aproach | | | |
| 1 | Öğretim elemanının ders notları | | | 2 | The Biology of plasmids | | | 3 | E.coli Plasmid Vectors | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 02.12.2023 | 2 | 50 | | End-of-term exam | 16 | 25.12.2023 | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 2 | 14 | 28 | | Arasınav için hazırlık | 3 | 8 | 24 | | Arasınav | 2 | 1 | 2 | | Kısa sınav | 2 | 14 | 28 | | Dönem sonu sınavı için hazırlık | 3 | 14 | 42 | | Dönem sonu sınavı | 2 | 1 | 2 | | Diğer 1 | 4 | 14 | 56 | | Total work load | | | 224 |
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