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| BIYI5210 | Recombinant DNA Technology | 3+0+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Prof. Dr. Ali Osman BELDÜZ | | Co-Lecturer | Prof: Dr. Sabriye ÇANAKÇI | | Language of instruction | | | Professional practise ( internship ) | None | | | | The aim of the course: | | The main objectives of this course are to provide the student with a clear presentation of the basic concepts and principles of related subjects. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | describe the structure of nucleic acids | 1,3,7,8 | 1,3,5, | | PO - 2 : | explain gene organisation and expression | 1,3,7,8 | 1,3,5, | | PO - 3 : | explain mutations, repair and replication of DNA | 1,3,7,8 | 1,3,5, | | PO - 4 : | isolate and safely handle nucleic acids in the laboratory | 1,3,7,8 | 1,3,5, | | PO - 5 : | explain principles of analyses methods of nucleic acids | 1,3,7,8 | 1,3,5, | | PO - 6 : | perform basic nucleic acid manipulations | 1,3,7,8 | 1,3,5, | | PO - 7 : | appreciate the role of genes in medicine and industry | 1,3,7,8 | 1,3,5, | | PO - 8 : | appreciate the applications and implications of recombinant DNA technology in medicine and industry | 1,3,7,8 | 1,3,5, | | PO - 9 : | demonstrate an ability to critically analyze experimental data | 1,3,7,8 | 1,3,5, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| DNA as basic genetic material, genetic code, elements that control gene expression. Engineering a recombinant DNA. Polymerase chain reaction. Moveable genes. In vitro mutagenesis. Gene transformation. Oncogenes. Genes that control Drosophila development. Importance of recombinant DNA for human genetics and biotechnology. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | DNA restriction, cloning and DNA sequencing | | | Week 2 | PCR, primer design and analysis where PCR are being used | | | Week 3 | cloning, hunting for correct clones and isolation of cloned genes | | | Week 4 | differantial hybridizations, hybridizations, chromosome walking and functional assays | | | Week 5 | usage of databases | | | Week 6 | in vitro mutagenesis | | | Week 7 | in vitro mutagenesis | | | Week 8 | Using Yeast to Study Eukaryotic Gene Function | | | Week 9 | Mid-term exam | | | Week 10 | Using Yeast to Study Eukaryotic Gene Function | | | Week 11 | Introduction of Forein Genes into Mice | | | Week 12 | Introduction of Forein Genes into Mice | | | Week 13 | Genetic Engineering of Plants, homework and project presentations | | | Week 14 | Genetic Engineering of Plants, homework and project presentations | | | Week 15 | Genetic Engineering of Plants, homework and project presentations | | | Week 16 | | | | |
| 1 | Recombinant DNA, James D. Watson, Michael Gilman, Mark Zoller, Jan Witkowski, 2nd Ed. Paperback, 626pp, ISBN: 0716722828, March 1992, W. H. Freeman Company. | | | |
| 1 | Lecture Notes | | | 2 | Molecular Biotechnology: Principles and Applications of Recombinant DNA, Bernard R. Glick, Jack J. Pasternak, and Cheryl L. Patten, 2nd Ed. Paperback, ISBN: 1-55581-136-1, ASM | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 23/11/2023 | 1,5 | 30 | | Presentation | 15 | 07/01//2024 | 1 | 10 | | Homework/Assignment/Term-paper | 15 | 07/01/2024 | 1 | 10 | | End-of-term exam | 16 | 17/01/2024 | 1,5 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 4.5 | 13 | 58.5 | | Arasınav için hazırlık | 6 | 7 | 42 | | Arasınav | 1.5 | 1 | 1.5 | | Ödev | 15 | 1 | 15 | | Kısa sınav | 2 | 7 | 14 | | Dönem sonu sınavı için hazırlık | 6 | 7 | 42 | | Dönem sonu sınavı | 2 | 5 | 10 | | Total work load | | | 225 |
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