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| ORM7264 | Plant Tissue Culture | 2+0+2 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Third Cycle | | Status | Elective | | Department | DEPARTMENT of FOREST ENGINEERING | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Group study, Lab work , Practical | | Contact Hours | 14 weeks - 2 hours of lectures and 2 hours of laboratory per week | | Lecturer | Prof. Dr. Ali Ömer ÜÇLER | | Co-Lecturer | Prof. Dr. Zeki YAHYAOGLU | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The course aims to provide the students with a general knowledge of plant tissue culture biotechnological method in plant production and tree breeding studying. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | define plant tissue culture as a plant production method | 3,5,7 | 1 | | PO - 2 : | use tissue culture in tree breeding | 3,5,7 | 5 | | PO - 3 : | learn preparing methods of media and media content used in tissue culture | 3,5,7 | 4 | | PO - 4 : | learn sterile working in laboratory and effective using of laboratory | 3,5,7 | 4 | | PO - 5 : | have knowledge about biotechnology. | 3,5,7 | 1 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Historical notes, tissue culture methods: anther culture, protoplast culture, callus culture, meristem culture, plant cell culture, embriyo culture. Applications area of plant tissue culture: production of virus-free plants, preservation of tissue culture, breeding, rapid propagation. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Introduction, Basic information for tissue culture | | | Week 2 | Application of Plant tissue culture, Biyotechnology and tissue culture, tissue culture in plant breeding | | | Week 3 | Laboratory facilities, laboratory units, laboratory equipment | | | Week 4 | Media formulas and components, chemicals, plant growth regulators etc. | | | Week 5 | Media preparation and sterilization, Liquid and solid media | | | Week 6 | Explants and their preparation, sterilization, resources of explant | | | Week 7 | Micropropagation methods, callus culture, meristem culture, anther culture, Laboratory experiment I | | | Week 8 | Mid-term exam | | | Week 9 | Cell culture, protoplast culture, protoplast isolation and fusion, embryo culture, somatic embryogenesis | | | Week 10 | Laboratory experiment II | | | Week 11 | Laboratory experiment III | | | Week 12 | Laboratory experiment IV | | | Week 13 | Laboratory experiment V | | | Week 14 | Laboratory experiment VI | | | Week 15 | Laboratory experiment VII | | | Week 16 | End-of-term exam | | | |
| 1 | Cell and Tissue Culture in Forestry, 1987; Volume I, J.M. Bonga and Don J. Durzan Editors, Martınus Nıjhoff Publishers. 422 p. | | | |
| 1 | Cell and Tissue Culture in Forestry, 1987; Volume II, J.M. Bonga and Don J. Durzan Editors, Martınus Nıjhoff Publishers. 447 p. | | | 2 | Cell and Tissue Culture in Forestry, 1987; Volume III, J.M. Bonga and Don J. Durzan Editors, Martınus Nıjhoff Publishers. 416 p. | | | 3 | Kyte, M. 1987. Plants from Test Tubes-An Introduction to Micropropagation, Timber Press. Portland, Oregon, 160 p. | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 25/11/2016 | 1 | 30 | | Laboratory exam | 13 | 13/12/2016 | 2 | 20 | | End-of-term exam | 16 | 05/01/2017 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 14 | 28 | | Sınıf dışı çalışma | 3 | 14 | 42 | | Laboratuar çalışması | 2 | 14 | 28 | | Arasınav için hazırlık | 2 | 7 | 14 | | Arasınav | 2 | 1 | 2 | | Uygulama | 2 | 14 | 28 | | Klinik Uygulama | 0 | 0 | 0 | | Ödev | 2 | 10 | 20 | | Proje | 2 | 9 | 18 | | Kısa sınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 2 | 8 | 16 | | Dönem sonu sınavı | 2 | 1 | 2 | | Diğer 1 | 2 | 8 | 16 | | Diğer 2 | 0 | 0 | 0 | | Total work load | | | 215 |
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